| Unlockable | How to Unlock |
| Chiisana Hoshi ga Oriru Toki AMV | Complete the Puzzle |
| Choosing Miyu w/ Alyssa | When selecting Miyu, place your selection box on Miyu and press and hold 'Select' then the 'O' button. (FB and Survival modes only.) |
| Extra Costume for Shiho and Akira | After obtaining their puzzle piece, play BR mode with them again and beat more than 7 foes in one game. Beating more foes improves your odds. |
| Obtaining a puzzle piece | Play BR mode and beat more than 7 foes in one game. The greater number of foes you beat, the better chances you have of getting the puzzle piece. |
| Obtaining the last extra Costume | Complete a character's sub-Child collection. (Fumi and Dark Mikoto do not have extra costumes to unlock.) |
| Selecting Dark Mikoto | Place the selection box on Mikoto and press and hold 'Select' then the 'O' button. Mikoto will switch to Dark Mikoto. |
| Senretsu!! System voices | Win at least 14 matches in Survival Mode with Shizuru, Yukino, Yukariko, Shiho, and Fumi to unlock their respective System Voices. |
| Unlocking 'Mai-HiME Bakurestu Characters and System Voices | Have a Bakurestu save game on your PSP. Go to Options: Convert. Choose 'Yes' (the two character option). |
| Unlocking Battle Royal and Survival | Complete Story mode once. |
| Unlocking Dark Mikoto | Win at least 14 matches in Survival Mode with Mikoto. Dark Mikoto's profile will also be unlocked. |
| Unlocking Pictures/Movies | Approximately half will become unlocked as you progress through Story mode. |
