| Unlockable: | How to Unlock: |
| Bear | Beat Onikage story mode |
| Echigoya | Beat special mission 'Tenchu' |
| Jinnai | Beat Tesshu story mode |
| Jonosuke | Beat Ayame story mode |
| Juzo | Beat Rin story mode |
| Kenpo Bozo | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Kunoichi | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Kuroya Jo-Kunoichi | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Kuroya Jonin | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Merchant | Beat special mission 'Tenchu' |
| Mifuyu | Beat Ayame story mode |
| Monk | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Mr. D | Beat special mission 'Illusion' |
| Ninja | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Ninja dog | Beat Onikage story mode |
| Onikage | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Policeman | Beat special mission 'Illusion' |
| Ranzo | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Ronin | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Salary-man samurai | Beat special mission 'Illusion' |
| Samurai | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Shimatsuya doctor | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Shimatsuya female | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Shimatsuya samurai | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Shimatsuya villager | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Shinogi | Beat Tesshu story mode |
| Sho | Beat Rikimaru, Ayame, Rin and Tesshu story modes |
| Tajima Eigoro | Beat Rin story mode |
| Tatsumaru | Beat Rikimaru story mode |
