| Unlockable: | How to Unlock: |
| EX Story Mode | Beat Story Mode on any difficulty |
| Flight of the S.S. Brass minigame | Beat EX Story on any difficulty as Mario |
| Hard Story Mode | Beat Normal Story Mode |
| Note Pickup minigame | Beat EX Story on any difficulty as Luigi |
| Super Hard Mode | Beat a song in Very Hard Mode without losing the dance meter |
| Unlock 10 minigames | Beat Story Mode as any character |
| Unlock 18 songs in Free Mode | Beat Story Mode |
| Unlock an additional 6 songs in Free Mode | Beat EX Story Mode |
| Unlock the minigame Coin Collection | Beat EX Story Mode |
| Unlock the minigame Treasure Boxes | Beat Story Mode (Or EX Story Mode) as Mario |
| Unlock the minigame Whee! | Beat Story Mode (Or EX Story Mode) as Luigi |
| Unlock the song Choir on the Green in Free Mode | Play EX Story Mode on any difficulty and buy the Secret Song in the shop in World 1 for 100 Coins |
| Unlock the song Hop, Mario! in Free Mode | Play EX Story Mode on any difficulty and buy the Secret Song in the shop in World 2 for 200 Coins |
| Unlock the song Piroli in Free Mode | Play EX Story Mode on any difficulty and buy the Secret Song in the shop in World 4 for 400 Coins |
| Unlock the song Wheres the Exit? in Free Mode | Play EX Story Mode on any difficulty and buy the Secret Song in the shop in World 3 for 300 Coins |
| Very Hard Story Mode | Beat Hard Story Mode |
